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将正常人外周血单核细胞(monocytes,Mon)与不同浓度的P物质(substance P,SP)共同温育24小时,借抗HLA-DR(Tü36,德国)和抗HLA-DQ(Tü22,德国)的单克隆抗体以碱性磷酸酶标记法(APAAP法),在光学显微镜下检测Mon表达HLA-DR和HLA-DQ抗原的情况。结果表明:高于血浆生理浓度的SP(10~(-10)mol/L,10~(-8)mol/L,10~(-6)mol/L)能明显地增加正常人外周血中HLA-DQ~+Mon的百分率(48.8±2.5%,54.5±2.6%,49.0±4.3%),与对照组者(37.4±1.7%)相比,分别为P<0.01,P<0.01,P<0.05,其中以10~(-8)mol/L SP的作用最强,而血浆生理浓度的SP(10~(-12)mol/L)则不能明显地增加HLA-DQ~+Mon的百分率(41.8±3.1%),与对照组者相比,P>0.05。在本实验条件下,上述浓度的SP对Mon膜HLA-DR抗原表达的影响不明显(P>0.05)。作者推测,在应激反应时,血中增多的SP可能借其对免疫反应的正调节作用而部分对消过多糖皮质激素的免疫抑制作用。
Normal monocytes (monocytes) were incubated with different concentrations of substance P (SP) for 24 hours. Anti-HLA-DR (Tü36, Germany) and anti HLA-DQ ) Was detected by the alkaline phosphatase-labeled method (APAAP method) under a light microscope to detect the expression of HLA-DR and HLA-DQ antigen by Mon. The results showed that SP (10 ~ (-10) mol / L, 10 ~ (-8) mol / L, 10 ~ (-6) mol / L) higher than the plasma concentration The percentages of HLA-DQ ~ + Mon (48.8 ± 2.5%, 54.5 ± 2.6%, 49.0 ± 4.3%) were significantly lower than those of the control group (37.4 ± 1.7%, P <0.01, P < 0.05, of which 10 -8 mol / L SP was the strongest, while plasma concentration of SP (10 ~ (-12) mol / L) did not significantly increase the percentage of HLA-DQ ~ + Mon 41.8 ± 3.1%), compared with the control group, P> 0.05. Under the experimental conditions, the above concentration of SP had no significant effect on the expression of HLA-DR antigen (P> 0.05). The authors speculate that in the stress response, increased blood SP may by virtue of its positive role in the immune response and the elimination of excess glucocorticoid immunosuppressive effect.