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目的建立同种异体肝细胞浆蛋白诱导的系统性红斑狼疮(SLE)兔模型。方法 20只雌性新西兰兔,随机分为对照组和模型组,10只/组,分别于第0天及第1、2、4、6、8、10、12周末耳缘静脉注射PBS及同种异体兔肝细胞浆总蛋白500μg(终质量浓度1 mg/ml),监测其肝肾功能、尿蛋白,并用酶联免疫吸附试验检测血ANA、抗dsDNA抗体和抗sm/nRNP抗体,通过HE染色及免疫组化观察肾脏及皮肤的组织病理变化。结果与对照组比较,模型组兔抗dsDNA抗体和抗sm/nRNP抗体高于对照组(P>0.05),80%的模型组动物出现皮肤及肾脏损害,并有尿蛋白升高、肌酐升高以及低蛋白血症。肾脏病理主要表现为肾小球系膜细胞增生,并伴有肾小管间质及血管病变,免疫组化示IgG系膜区沉积。皮肤病理表现为基底细胞液化变性。结论应用肝脏细胞浆蛋白可成功诱导SLE兔模型,该模型具有显著SLE样皮肤及肾脏的损害,可作为SLE研究的良好工具。
Objective To establish a rabbit model of systemic lupus erythematosus (SLE) induced by allogeneic hepatocyte plasma protein. Methods Twenty female New Zealand white rabbits were randomly divided into control group, model group and 10 rats in each group. Rabbits were injected with PBS on the 0th and 1st, 2nd, 4th, 6th, 8th, Hepatic and renal function, urinary protein were detected in allogeneic rabbit hepatocyte plasma total protein 500μg (final concentration of 1 mg / ml), and blood ANA, anti-dsDNA antibody and anti-sm / nRNP antibody were detected by enzyme-linked immunosorbent assay The histopathological changes of kidney and skin were observed by immunohistochemistry. Results Compared with the control group, the anti-dsDNA antibody and anti-sm / nRNP antibody in the model group were higher than those in the control group (P> 0.05). 80% of the model group had skin and kidney damage and increased urinary protein and creatinine As well as hypoproteinemia. Kidney pathology showed mainly mesangial cell proliferation, accompanied by tubulointerstitial and vascular lesions, immunohistochemistry showed deposition of IgG mesangial area. Skin pathology showed basal cell liquefaction degeneration. Conclusion SLE rabbit model can be successfully induced by using liver cytoplasm protein. This model has significant damage to SLE-like skin and kidney, and can be used as a good tool for SLE study.