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A new forsythenside,4-hydroxy-4-[8-[[1-[(4-hydroxyphenyl)acetyl]-β-D-glucopymllosyl-6-]oxy]ethyl]-2,5-cyclohexadien-1-one,named forsythenside F,wasisolasted from the plant of Forsythia suspensa(Thunb.)Vahl.The structure of the new compound was established on the basis of various spectroscopic analysis,including 1H NMR.13C NMR,2D NMR techniques(HMBC and HSQC).and HR-ESI-MS.