论文部分内容阅读
目的研究丹参酮ⅡA对大鼠体内茶碱药动学特征的影响。方法将24只大鼠随机分成3组,Ⅰ组:生理盐水,ip×7 d;Ⅱ组:丹参酮ⅡA 7 mg·kg?1·d?1,ip×7 d;Ⅲ组:丹参酮ⅡA 20 mg·kg?1·d?1,ip×7 d。于第7天给药后3组均灌胃给予氨茶碱(20.8 mg·kg?1),分别于给药前后的0.17,0.33,0.5,1.0,1.5,2.0,4.0,6.0,8.0,10.0,12.0 h尾静脉采血。用HPLC测定血浆中茶碱的血药浓度,并用DAS计算药动学参数。结果茶碱在大鼠体内主要药动学参数,Cmax为(29.43±1.53)mg·L?1、AUC0→∞为(156.95±10.22)mg·h·L?1、MRT0-∞为(4.44±0.42)h、CLz/F为(0.13±0.01)L·h?1·kg?1。与Ⅰ组比较,Ⅱ组各参数无显著性差异,Ⅲ组主要参数AUC0→12h、AUC0→∞、Cmax、MRT0-t、MRT0-∞、CLz/F等有显著性差异(P<0.05),其中Cmax、AUC0→∞分别下降了7.54%和14.69%,CLz/F增加了23.08%。结论高剂量的丹参酮ⅡA能影响茶碱在大鼠体内药动学特征,而低剂量无影响。
Objective To study the effect of tanshinone ⅡA on pharmacokinetics of theophylline in rats. Methods Twenty-four rats were randomly divided into 3 groups: group Ⅰ: saline, ip × 7 d; group Ⅱ: tanshinone Ⅱ A 7 mg · kg -1 · d -1, ip × 7 d; group Ⅲ: tanshinone Ⅱ A 20 mg · Kg · 1 · d · 1, ip × 7 d. Aminophylline (20.8 mg · kg -1) was administered intragastrically on the 7th day after administration, respectively, before and after administration of 0.17,0.33,0.5,1.0,1.5,2.0,4.0,6.0,8.0,10.0 , 12.0 h tail vein blood collection. Plasma concentrations of theophylline in plasma were determined by HPLC and pharmacokinetic parameters were calculated by DAS. Results The main pharmacokinetic parameters of theophylline in rats were Cmax (29.43 ± 1.53) mg · L -1, AUC0 → ∞ (156.95 ± 10.22) mg · h · L -1 and MRT0-∞ (4.44 ± 0.42) h, CLz / F was (0.13 ± 0.01) L · h -1 · kg -1. Compared with group Ⅰ, there was no significant difference between group Ⅱ and group Ⅲ (P <0.05). The main parameters AUC0 → 12h, AUC0 → ∞, Cmax, MRT0-t, MRT0-∞ and CLz / Among them, Cmax, AUC0 → ∞ decreased by 7.54% and 14.69% respectively, CLz / F increased by 23.08%. Conclusion High dose of tanshinone Ⅱ A can affect the pharmacokinetics of theophylline in rats, but no effect of low dose.