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本文选用苯并(a)比对FADU法(DNA解螺旋荧光分析法)在检测环境间接致突变剂DNA链断裂效应上的应用进行了初步探讨。体外试验结果显示,在37℃条件下与人外周血白细胞共同作用60分钟后,经代谢活化的苯并(a)芘可造成白细胞DNA链的断裂明显增多,在10~(-7)～10~(-4)M染毒剂量下,单
In this paper, the application of benzo (a) ratio FADU method (DNA unwinding fluorescence analysis) in the detection of environment-induced DNA strand breaks mutagenic effect was initially discussed. In vitro experiments showed that the metabolic activation of benzo (a) pyrene caused a significant increase in the cleavage of the leucocyte DNA chain after co-treatment with human peripheral blood leukocytes at 37 ° C for 60 minutes. After 10 ~ (-7) ~ 10 ~ (-4) M dose, single