20名志愿者平均年龄43岁,均无百日咳病史,也未接种过百日咳菌苗.选择标准是志愿者百日咳毒素(PT)中和抗体水平<16.所用菌苗为JNIH-7纯化类毒素,每针含10～15μg蛋白氮.左上臂皮下深部注射,每针2.5ml.免疫前8～33天第1次静脉采血,15名志愿者免疫后14～23天第2次采血,13名免疫后32个月第3次采血.中和抗体用 Twenty volunteers, mean age 43 years, had no history of pertussis and had not been vaccinated against pertussis B. The criteria for selection were the level of neutralizing antibody to pertussis toxin (PT) in volunteers <16. The vaccine used was a purified toxoid for JNIH-7, Each needle contains 10 ~ 15μg of protein nitrogen.The left upper arm subcutaneous deep injection, 2.5ml per needle.The first vein blood 8 to 33 days before immunization, 15 volunteers 14 to 23 days after the second immunization blood 12, 13 immunization After 32 months of the third blood collection. Neutralizing antibody