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菠萝叶主要含酚类成分,具有抗氧化和调血脂作用。其主要成分为对香豆酸。该文以对香豆酸为主要测试成分,观察了菠萝叶酚类成分中对香豆酸体外人源肝HepG2细胞跨膜转运的特点及其对糖脂负荷细胞实验中葡萄糖,总胆固醇和甘油三醇的转运代谢的影响。结果表明,菠萝叶酚具有较好的跨膜转运特点。给药后5 min,菠萝叶酚中的对香豆酸即细胞内达到较高的浓度,60 min达峰值。具有时间和剂量依赖性。在促进葡萄糖转运体(GLUT4)和低密度脂蛋白受体(LDLR)表达的同时,菠萝叶酚能够降低细胞内的糖脂含量。这种降低细胞内脂含量的作用与促进脂蛋白脂酶(LPL)、肝甘油三酯脂酶(HTGL)表达有关,降低细胞内葡萄糖含量的作用与促进肝糖原合成有关。
Pineapple leaves contain phenolic compounds, with anti-oxidation and blood lipid-lowering effect. Its main component is p-coumaric acid. In this paper, p-coumaric acid was used as the main test component to observe the transmembrane transport of human acid HepG2 cells to coumaranic acid in vitro and its effect on glucose, total cholesterol and glycerol Effect of triol on transport metabolism. The results showed that pineapple leaf phenol has better transmembrane transport characteristics. At 5 min after administration, p-coumaric acid in pineapple leaf phenol reached a higher intracellular concentration and reached a peak at 60 min. With time and dose-dependent. While promoting the expression of glucose transporter (GLUT4) and low density lipoprotein receptor (LDLR), pineapple leaf phenolics can reduce intracellular glycolipid content. The effect of reducing intracellular lipid content is related to the promotion of the expression of lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL), and the effect of reducing intracellular glucose content is related to the promotion of glycogen synthesis.