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目的:探讨人睾丸基因TDRG1在睾丸肿瘤组织中的蛋白表达及其病理学意义。方法:运用组织芯片技术、免疫组化法检测人睾丸特异基因TDRG1在睾丸肿瘤组织和正常睾丸组织中的表达。结果:15例正常人睾丸对照组中11例(73.3%)TDRG1蛋白表达为阳性;26例精原细胞瘤中7例(26.9%)TDRG1蛋白表达为阳性,7例畸胎瘤中4例(57.1%)TDRG1蛋白表达为阳性。而12例胚胎癌中10例(83.3%)TDRG1蛋白表达为阳性,10例卵黄囊瘤中8例(80.0%)TDRG1蛋白表达为阳性。精原细胞瘤实验组与正常人睾丸对照组相比较,两组间具有极显著性差异(P<0.01)。畸胎瘤实验组与正常人睾丸对照组相比较,两组间具有显著性差异(P<0.05)。而胚胎癌组和卵黄囊瘤组与正常人睾丸对照组相比较,没有显著性差异(P>0.05)。结论:TDRG1蛋白在精原细胞瘤和畸胎瘤的表达水平较正常对照睾丸组织显著降低,TDRG1可能为候选的抑癌基因。
Objective: To investigate the protein expression of human testis gene TDRG1 in testicular tumor and its pathological significance. Methods: Tissue microarray technique was used to detect the expression of human testis-specific gene TDRG1 in testicular tumor tissue and normal testicular tissue. Results: TDRG1 protein expression was positive in 11 cases (73.3%) in 15 normal testes control group, 7 cases (26.9%) in 26 cases of seminoma were positive, and 7 cases of teratoma in 4 cases ( 57.1%) TDRG1 protein expression was positive. TDRG1 protein expression was positive in 10 of 12 (83.3%) embryos and 8 (80.0%) of 10 yolk sac tumors. The difference between the two groups was significant (P <0.01). Teratoma experimental group compared with the normal testis control group, there was a significant difference between the two groups (P <0.05). There was no significant difference between embryonic carcinoma group and yolk tumor group and normal human testis group (P> 0.05). CONCLUSION: The expression of TDRG1 protein in seminoma and teratoma is significantly lower than that in normal control testes. TDRG1 may be a candidate tumor suppressor gene.