论文部分内容阅读
目的探讨脓毒症发病过程中T淋巴细胞亚群变化及其临床意义。方法选取医院2010年1月—2013年1月间收治的脓毒症患者62例设为观察组,设同期非脓毒症的危重症患者60例为对照组,采用流式细胞仪检测2组患者24 h外周血CD3+(CD3阳性淋巴细胞)、CD4+(CD4阳性淋巴细胞)、CD8+(CD8阳性淋巴细胞)的百分比,及CD4+/CD8+值改变情况,研究数据采用SPSS 12.0统计软件处理,以评价机体细胞免疫功能变化情况。结果观察组外周血CD3+、CD4+及CD4+/CD8+值均低于对照组,组间比较差异有统计学意义(P<0.05);观察组中死亡患者外周血CD4+、CD8+及CD4+/CD8+值均低于存活组,组间比较差异有统计学意义(P<0.05)。结论脓毒症患者处周血CD3+、CD4+及CD4+/CD8+值均显著降低,且死亡组患者上述指标显著低于存活组,表明脓毒症患者存在免疫功能损害,且外周血CD4+、CD8+及CD4+/CD8+值愈低,提示患者预后不佳。
Objective To investigate the changes of T lymphocyte subsets during the pathogenesis of sepsis and its clinical significance. Methods Sixty-two patients with sepsis admitted to our hospital from January 2010 to January 2013 were selected as the observation group. Sixty critically ill patients with the same period of non-sepsis were selected as the control group. Flow cytometry was used to detect two groups The percentage of CD3 + (CD3 + lymphocytes), CD4 + (CD4 +) and CD8 + (CD8 +) lymphocytes in peripheral blood of patients at 24 h and the changes of CD4 + / CD8 + were analyzed by SPSS 12.0 statistical software to evaluate Body cellular immune function changes. Results The levels of CD3 +, CD4 + and CD4 + / CD8 + in the peripheral blood of the observation group were significantly lower than those of the control group (P <0.05). The CD4 +, CD8 + and CD4 + / CD8 + In survival group, there was significant difference between groups (P <0.05). Conclusions The levels of CD3 +, CD4 + and CD4 + / CD8 + in peripheral blood of patients with sepsis were significantly lower than those of the survivors, indicating that immune function was impaired in patients with sepsis. The levels of CD4 +, CD8 + and CD4 + / CD8 + value is lower, suggesting that patients with poor prognosis.