对组织学类型已经明确的冻存肿瘤细胞切片,采用改进的显微解剖技术.切取小至1mm、包含不过200个细胞的肿瘤小病灶,不必另外进行特殊的mRAN分离和纯化,即可获得足够的肿瘤mRNA,采用RT-PCR技术,成功进行β2微球蛋白、p21~(wall)和BRCA1基因cDNA片段的扩增。 主要步骤是:用恒冷切片机切聚20μm和40μm厚的乳腺癌组织切片,短暂染包后用水漂洗,再放入顶冷的Eppendorf试管内,低温下操作,并尽早 Tissue-typed frozen tumor cell sections have been developed using improved microdissection techniques.A small tumor lesion as small as 1 mm, containing less than 200 cells, can be excised and sufficient additional mRAN isolation and purification can be obtained The cDNA of β2 microglobulin, p21 wall and BRCA1 gene was successfully amplified by RT-PCR. The main steps are: cutting 20μm and 40μm thick breast tissue sections with a cryostat, briefly dyed and rinsed with water, placed in a top-cold Eppendorf tube and operated at low temperature