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背景:次声暴露导致大鼠血-视网膜屏障通透性增加。但由于视网膜微血管内皮细胞来源困难,关于其屏障损伤的离子机制报道较少。目的:探讨次声对视网膜微血管内皮细胞钙激活钾通道的影响。设计:完全随机实验对照的开放性研究。地点和材料:实验在第四军医大学航空临床教研室膜片钳实验室进行,实验对象为培养牛视网膜微血管内皮细胞。干预:取传代的牛视网膜微血管内皮细胞8Hz,130dB次声暴露0.5h。主要观察指标:视网膜微血管内皮细胞钙激活钾通道的活动情况。结果:8Hz,130dB次声暴露0.5h后,视网膜微血管内皮细胞KCa通道活性增加,暴露后置于孵箱内0.5h再行膜片钳离子电流的检测,则离子通道的活性也有所下降。结论:次声通过增加视网膜微血管内皮细胞钙激活钾通道的活性,导致膜去极化,引起钙离子进入细胞,内皮细胞收缩,造成一定程度的血-视网膜屏障通透性的损害。
Background: Infrasound exposure leads to increased blood-retinal barrier permeability in rats. However, due to the difficulty of deriving retinal microvascular endothelial cells, few reports have been reported on the ionic mechanism of barrier damage. Objective: To investigate the effect of infrasound on calcium-activated potassium channels in retinal microvascular endothelial cells. Design: A completely randomized, controlled, open study. Location and Materials: Experiments were carried out in the Patch Clamp Laboratory of Aviation Clinical Teaching and Research Institute of the Fourth Military Medical University. The experimental subjects were bovine retinal microvascular endothelial cells. Intervention: Take the passage of bovine retinal microvascular endothelial cells 8Hz, 130dB infrasound exposure 0.5h. MAIN OUTCOME MEASURES: Activity of calcium-activated potassium channels in retinal microvascular endothelial cells. Results: The activity of KCa channel in retinal microvascular endothelial cells increased after 0.5 h of 8 Hz and 130 dB infrasound exposure. After exposure for 0.5 h, the iontophoretic activity of ion channels was also decreased. CONCLUSION: Infrasound increases membrane-activated calcium channel activity in retinal microvascular endothelial cells, causing membrane depolarization, causing calcium ions to enter cells and endothelial cells to contract, resulting in a degree of blood-retinal barrier permeability damage.