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目的观察血管紧张素Ⅱ(AngⅡ)对体外培养孕12d小鼠肾组织中各期肾小体细胞增殖的影响。方法孕12d母鼠随机分为体外培养对照组和施加不同浓度AngⅡ(10-9mol/L～10-5mol/L)组。应用体外培养、免疫组织化学及免疫荧光技术,观察各组肾组织培养2、4、6d后各期肾小体增殖细胞核抗原(PCNA)的表达。结果AngⅡ能促进小鼠肾小体细胞的增殖,在一定范围内,AngⅡ浓度越大肾小体细胞增殖越明显,且与作用时间呈正相关;培养4d的肾组织比培养2d的肾组织增殖明显。结论AngⅡ对发育期小鼠肾小体细胞有促增殖作用,且此作用有浓度时间依赖性。
Objective To observe the effects of Ang Ⅱ on the proliferation of renal mesangial cells of pregnant mice in vitro. Methods The pregnant 12d female rats were randomly divided into in vitro culture control group and Ang Ⅱ (10-9mol / L ~ 10-5mol / L) group. In vitro culture, immunohistochemistry and immunofluorescence techniques were used to observe the expression of proliferating cell nuclear antigen (PCNA) in each group after 2,4,6 days of culture. Results AngⅡcan promote the proliferation of mouse renal somatic cells. Within a certain range, the greater the concentration of AngⅡ, the more obvious the proliferation of renal cells was, and it was positively correlated with the action time. The renal tissue cultured 4d had more proliferation than the 2d . Conclusion Ang Ⅱ can promote the growth of mouse renal somatic cells in a concentration-dependent manner.