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多酶片中胰酶的活力,现仍规定按1977年版《中国药典》中的酪蛋白转化力测定法测定,因此多酶片原料之一的高倍胰酶的活力,也仍按相应的方法测定。长期从事化验工作中感到,该方法有关操作过程规定中有一段不够合理即从“取比色管6支,分别加入上述溶液……并用水稀释,使各成5ml,置40°水浴中,候管内温度升至40℃时,各管中分别加40℃的酪蛋白溶液5ml”止,是不合理的,主要因为供试品是酶,它的水溶液是不稳定的,特别是在不适的pH条件下,受热过程中活力损失更大,当置40℃水浴中,候管内温度达到40℃的升温过程中,活力就有损失,升温过程的长短与水浴大
The activity of trypsin in multi-enzyme tablets is still regulated by the determination of casein conversion in the 1977 edition of “Chinese Pharmacopoeia”, so the activity of high-power pancreatin in one of the multi-enzyme tablets is still determined by the corresponding method . Engaged in laboratory work for a long time that the method of operation of the provisions of a section is not reasonable enough from the "color tube 6, were added to the above solution ... and diluted with water, so that each 5ml, set 40 ° water bath, When the temperature in the tube rose to 40 ° C, it was unreasonable to add 5 ml of casein solution to each tube at 40 ° C, mainly because the test substance was an enzyme whose aqueous solution was not stable, especially at an uncomfortable pH Under the conditions, the loss of vitality during the heating process is greater, when set at 40 ℃ water bath, the waiting tube temperature reaches 40 ℃ during the heating process, the loss of energy, the length of the heating process and the water bath large