论文部分内容阅读
用原发性肝癌患者手术切除的肝癌组织的 DNA 和肝癌患者、肝癌患者一级亲属与对照组个体白血细胞的 DNA 以及人工流产胎儿绒毛的 DNA 分别经限制性核酸内切酶 Eco RI 酶解、琼脂糖凝胶电泳分离、Southern 转移、并用~(32)P-标记的癌基因 N-ras 探针作分子杂交,以观察其 RFLPs。结果在中国汉族人基因组癌基因 N-ras 的左段意外地发现4.0kbp,7.8kbp,12.0kbp 等3个未见报告的特异等位片段,而未发现已见报告的8.8kbp,9.0kbp 或9.2kbp 的片段;在该基因的右段则除已见报告的7.0kbp 片段外还获得1个未见报告的12.8kbp 的等位片段。未探查出这些 RFLPs 与肝癌易感性的相关性。
DNA of primary liver cancer patients with liver cancer and liver cancer patients, first-degree relatives of patients with liver cancer and control group white blood cells and human abortion fetus DNA were restriction endonuclease Eco RI digestion, Agarose gel electrophoresis, Southern transfer, and ~ (32) P-labeled oncogene N-ras probe for molecular hybridization to observe its RFLPs. RESULTS: Three unidentified allelic fragments of 4.0kbp, 7.8kbp and 12.0kbp were unexpectedly found on the left of Chinese Han Chinese oncogene N-ras, while the reported reported 8.8kbp, 9.0kbp or 9.2kbp fragment; in the right part of the gene in addition to the reported 7.0kbp fragment also received an unreported 12.8kbp allelic fragment. No correlation between these RFLPs and susceptibility to liver cancer has been explored.