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温度在２６～３４℃范围内，甘薯茎尖分生组织培养均可成苗。在此温度范围以外，因品种不同成苗率有所差别。培养适宜温度为２８～３０℃。２２～２４℃条件下，甘薯茎尖分生组织虽能生长，但不能成苗。培养基ｐＨ值４６～７０对较易培养成苗的甘薯品种成苗率无明显影响，但ｐＨ值过高对较难培养成苗的品种有影响。培养基琼脂浓度０４％～０７％均适于甘薯茎尖分生组织培养。
The temperature in the range of 26 ~ 34 ℃, sweet potato shoot tip meristem culture can be seedlings. In this temperature range, due to different varieties into different seedling rate. The optimum culture temperature is 28 ~ 30 ℃. Under the condition of 22 ~ 24 ℃, although the shoot apical meristem of sweet potato can grow, it can not become a seedling. The pH values of medium 4 6 ~ 7 0 had no obvious effect on the seedling rate of sweet potato varieties, but the high pH value had an impact on the varieties which were more difficult to cultivate. Medium agar concentration of 0 4% ~ 0 7% are suitable for sweet potato shoot tip meristem culture.