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通过对婴儿外周血(PB)、肠系膜淋巴结(MLN)和肠上皮内(IE)来源淋巴细胞的比较性研究,以分析婴儿肠道HIV-1感染容许性和HIV-1复制支持性的细胞与分子基础。分离婴儿外周血单个核细胞(PBMC),肠系膜淋巴结淋巴细胞(MLNL)和肠上皮间淋巴细胞(iIEL),制备单细胞悬液,运用流式细胞术结合荧光抗体染色技术:(1)检测不同部位的CD3+T细胞中CD4+辅助性T细胞和CD8+杀伤性T细胞比例;(2)检测不同部位的CD4+T细胞CCR5和CXCR4以及CD69和HLA-DR的表达水平。结果发现:(1)iIEL中的CD4+辅助性T细胞占CD3+T细胞的比例明显低于PBMC和MLNL,而CD8+杀伤性T细胞占CD3+T细胞的比例明显高于PBMC和MLNL;(2)iIEL中CD4+T细胞CCR5和CXCR4以及CD69和HLA-DR的表达水平均明显高于PBMC和MLNL。结果表明:婴儿iIEL中CD4+T淋巴细胞与PBMC和MLNL的CD4+T淋巴细胞相比较,高表达HIV-1入胞共受体CCR5和CXCR4,提示iIEL更易被HIV-1感染;iIEL中CD4+T淋巴细胞高表达细胞活化标记分子CD69和HLA-DR,说明其更有利于HIV-1复制,这种高基础活化率可能与其不断接触小肠来源的食物和共生菌抗原有关。由此可见,婴儿肠道具有了HIV-1感染容许性和HIV-1复制支持性的细胞和分子基础,因此成了HIV-1最易感的解剖部位,这可能是围产期HIV感染的婴儿病毒载量明显高于成年人,且病情进展也比成年人快的原因。
A comparative study of infants with peripheral blood (PB), mesenteric lymph nodes (MLN) and intra intestinal epithelial (IE) -derived lymphocytes was conducted to analyze the relationship between infant gut HIV-1 infection tolerance and HIV-1 replication-supporting cells and Molecular basis. Isolation of infant peripheral blood mononuclear cells (PBMC), mesenteric lymph node lymphocytes (MLNL) and intestinal epithelial lymphocytes (iIEL), prepared single cell suspension, using flow cytometry and fluorescent antibody staining techniques: (1) (2) To detect the expression levels of CCR5 and CXCR4 and CD69 and HLA-DR of CD4 + T cells in different parts of the CD3 + T cells. The results showed that: (1) The proportion of CD4 + helper T cells to CD3 + T cells in iIEL was significantly lower than that in PBMCs and MLNLs, while the proportion of CD8 + killer T cells to CD3 + T cells was significantly higher than PBMCs and MLNLs; ) The expression levels of CCR5 and CXCR4, CD69 and HLA-DR of CD4 + T cells in iIEL were significantly higher than those in PBMC and MLNL. The results showed that compared with CD4 + T lymphocytes in PBMC and MLNL, CD4 + T lymphocytes in infants with high expression of HIV-1 CCR5 and CXCR4, suggesting that iIEL is more susceptible to HIV-1 infection; iIEL CD4 + T lymphocyte-overexpressing cell activation marker molecules CD69 and HLA-DR, indicating that it is more conducive to HIV-1 replication, this high basal activation rate may be related to its continuous exposure to small intestine-derived foods and symbiotic antigens. Thus, the infant’s gut has the cellular and molecular basis for the admissibility of HIV-1 infection and the supportive HIV-1 replication and therefore has become the most susceptible anatomy for HIV-1, which may be perinatal HIV infection Infant viral load was significantly higher than that of adults, and the condition progressed faster than adults.