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有表达ＧＤＮＦ的重组腺病毒直接感染中脑多巴胺能神经元来源的ＭＮ９Ｄ细胞并经神经毒素ＭＰＰ＋处理．感染３６ｈ分别收获细胞及其培养基，用反相高效液相色谱方法测定多巴胺含量．结果显示，经ＧＤＮＦ重组腺病毒感染的ＭＮ９Ｄ细胞内及其培养基中的多巴胺水平分别增加５０．７％和５３．５％．给予神经毒素ＭＰＰ＋损伤后，细胞内多巴胺含量降低５３．５％，但同时给予ＧＤＮＦ腺病毒则可抑制这种降低，并使多巴胺水平增加１４１．３％．以上结果提示ＧＤＮＦ腺病毒可提高细胞内的多巴胺水平并促进其释放，同时还具有对抗神经毒素ＭＰＰ＋损伤作用，表明ＧＤＮＦ重组腺病毒用于帕金森氏病基因治疗具有良好的前景．
The recombinant adenovirus expressing GDNF directly infects the midbrain dopaminergic neuron-derived MN9D cells and is treated with neurotoxin MPP +. 36h infected cells and their culture medium were harvested, the determination of dopamine content by reverse-phase high performance liquid chromatography. The results showed that dopamine levels in MN9D cells infected with GDNF recombinant adenovirus and their culture medium increased by 50.7% and 53.5%, respectively. After administration of neurotoxin MPP +, intracellular dopamine levels decreased by 53.5%, while administration of GDNF adenovirus suppressed this reduction and increased dopamine levels by 141.3%. These results suggest that GDNF adenovirus can increase intracellular levels of dopamine and promote its release, as well as protect against MPP +, indicating that GDNF recombinant adenovirus has good prospects for gene therapy of Parkinson’s disease.