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AIM:To investigate the association of TNF polyrnorphisrnswith chronic atrophic gastritis (CAG) and gastric adenocarcin-oma in Chinese Han patients.METHODS:The TNFa-e 5 microsatellites and 3 RFLP siteswere typed using PCR technique,followed by high-voltagedenaturing PAGE with silver staining and restriction enzymedigestion respectively in specimens from 53 patients withCAG and 56 patients with gastric adenocarcinorna and164 healthy controls.The PCR products were cloned andsequenced.RESULTS:The frequency of TNF-β Ncol~*1/2 genotype washigher in patients with chronic atrophic gastritis than inhealthy controls,but no significant difference was observed(60.38% vs 46.34%,P=0.076).The frequency of TNa10allele was significantly higher in patients with chronicatrophic gastritis than in healthy controls (19.81% vs 11.89%,P=0.04).However,it did not relate to age,gender,atrophicdegree or intestinal metaplasia in patients with chronicatrophic gastritis.The frequency of TNF-β Ncol~*1/2 andd2/d6 genotypes were significantly higher in patients withgastric adenocarcinorna than in healthy individuals(P>0.05).However,TNF-β Ncol~*1/2 and d2/d6 genotypes did not relateto age,gender,grade of differentiation and clinicopathologicstage in patients with gastric adenocarcinorna.The frequencyof TNFa6bSc1 haplotype hornozygote was significantlylower in patients with gastric adenocarcinoma than inhealthy controls (1.79% vs 15.85%,P=0.006).CONCLUSION:TNFa10 allele may be a risk factor for chronicatrophic gastritis.TNF-β Ncol~*1/2 and d2/d6 genotypes areassociated with the susceptibility to gastric adenocarcinoma,whereas TNFa6b5c1 haplotype hornozygote may contributeto the resistance against gastric adenocarcinoma.
AIM: To investigate the association of TNF polyrnorphism with chronic atrophic gastritis (CAG) and gastric adenocarcin- oma in Chinese Han patients. METHODS: The TNFa-e 5 microsatellites and 3 RFLP siteswere typed using PCR technique, followed by high-voltagedenaturing PAGE with silver staining and restriction enzym- omyexpectively in specimens from 53 patients with CAG and 56 patients with gastric adenocarcinorna and 164 healthy controls. PCR products were cloned and sequenced .RESULTS: The frequency of TNF-βNcol ~ * 1/2 genotype washigher in patients with chronic atrophic gastritis (60.38% vs 46.34%, P = 0.076). The frequency of TNa10allele was significantly higher in patients with chronic atrophic gastritis than in healthy controls (19.81% vs 11.89%, P = 0.04). However, it did not relate to age, gender, atrophic degree or intestinal metaplasia in patients with chronic gastritis. The frequency of TNF-β Ncol ~ * 1/2 and d2 / d6 ge notypes were significantly higher in patients with gastric adenocarcinorna than in healthy individuals (P> 0.05) .However, TNF-βNcol ~ * 1/2 and d2 / d6 genotypes did not relate to age, gender, grade of differentiation and clinicopathologic staging in patients with gastric adenocarcinorna.The frequency of TNFa6bSc1 haplotype hornozygote was significantlylower in patients with gastric adenocarcinoma than inhealthy controls (1.79% vs 15.85%, P = 0.006) .CONCLUSION: TNFa10 allele may be a risk factor for chronic gastritis.TNF- β Ncol ~ * 1 / 2 and d2 / d6 genotypes areassociated with the susceptibility to gastric adenocarcinoma, whereas TNFa6b5c1 haplotype hornozygote may contribute to the resistance against gastric adenocarcinoma.