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以鸭梨(Pyrus.bretschneideri Rehd.cv.‘Yali’)多倍体(2×、3×、4×)为材料,应用甲基化敏感扩增多态性(methylation sensitive amplified polymorphism,MSAP)技术研究不同倍性鸭梨基因组DNA甲基化水平及模式变化.共筛选22对引物,平均每对引物产生约50条带,统计100～500 bp的扩增带,共计954个位点.分析表明:鸭梨2×、3×、4×分别检测到831、886、852个位点,发生甲基化位点分别占15.5%、20.0%、16.8%,其中全甲基化占9.7%、11.9%、9.7%.分析这些位点甲基化模式表明:甲基化模式在三个倍性间类型相同的占所检测位点的69.2%,仅两种倍性相同的为25.4%,各不相同的占5.5%,其中三倍体41.4%甲基化位点发生改变,显示出独特的DNA甲基化特点.
Using polyploid (2 ×, 3 ×, 4 ×) lines from Pyrus brevicaulei Rehd.cv.’Yali ’, the methylation sensitive amplified polymorphism (MSAP) A total of 22 pairs of primers were screened, with an average of about 50 bands per primer pair and a total of 954 bands with a total of 954 loci ranging from 100 to 500 bp : 831,886,852 sites were detected in 2 ×, 3 ×, 4 ×, respectively. The methylation sites accounted for 15.5%, 20.0%, 16.8%, respectively, of which, the methylation accounted for 9.7%, 11.9 %, 9.7% .Analysis of methylation patterns of these sites showed that the methylation pattern accounted for 69.2% of the detected sites with the same type among the three ploidies, and only 25.4% of the two ploidy identities, none of which The same accounted for 5.5%, of which triploid 41.4% methylation changed, showing a unique DNA methylation characteristics.