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目的研究医院临床分离及环境检出的肺炎克雷伯菌对消毒剂抗性水平和抗药基因携带的关系。方法采用最低抑菌浓度(MIC)、最小杀菌浓度(MBC)、聚合酶链反应(PCR)等试验方法,分析临床分离及环境中检出的肺炎克雷伯菌qacE△1-sulⅠ基因携带和对消毒剂的抗性。结果 10株肺炎克雷伯菌中有2株临床分离的多药耐药菌qacE△1-sulⅠ基因为阳性,8株环境检出菌株均为阴性;三氯异氰尿酸对10株肺炎克雷伯菌的MIC、MBC值均为500～1000mg/L,标准菌株为500mg/L;聚乙烯吡咯烷酮碘对10株肺炎克雷伯菌的MIC值为200～800mg/L,标准菌株为400mg/L;戊二醛对10株肺炎克雷伯菌的MIC值为1250～2500mg/L,标准菌株为1250mg/L。结论临床分离的肺炎克雷伯菌qacE△-sulⅠ基因携带率高于环境检出菌株,部分抗药基因阳性菌株对消毒剂产生抗性。
Objective To study the relationship between clinical isolates and environmental detection of Klebsiella pneumoniae to the level of disinfectant resistance and resistance gene carrier. Methods The clinical isolates and environments of Klebsiella pneumoniae qacE △ 1-sul I gene were detected by MIC method, MBC method and polymerase chain reaction (PCR) Resistance to disinfectants. Results Of the 10 strains of Klebsiella pneumoniae, 2 strains of multidrug-resistant bacteria qacE △ 1-sul I gene were positive, and 8 strains were negative. Trichloroisocyanuric acid The MIC and MBC values of the primary bacteria were 500-1000 mg / L and the standard strains were 500 mg / L respectively. The MIC value of polyvinylpyrrolidone iodine against 10 strains of K. pneumoniae was 200-800 mg / L and the standard strain was 400 mg / L The MIC value of glutaraldehyde to 10 strains of Klebsiella pneumoniae was 1250 ~ 2500 mg / L and the standard strain was 1250 mg / L. Conclusions The qacE △ -sulⅠ gene carrier rate of Klebsiella pneumoniae isolated clinically is higher than that of the isolates detected by environmental tests. Some strains resistant to Klebsiella pneumoniae are resistant to disinfectants.