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目的了解＋ＧＺ重复暴露后大鼠脑组织ｃ－ｆｏｓ和ｃ－ｊｕｎ基因表达的变化，探讨＋ＧＺ引起脑损害的分子机制，为＋ＧＺ防护研究提供实验依据。方法２４只雄性Ｗｉｓｔａｒ大鼠随机分成对照组、＋ＧＺ重复暴露后３０ｍｉｎ、６ｈ和２４ｈ四组，每组６只。对照组大鼠Ｇ值为＋１ＧＺ，实验组大鼠在动物离心机上经历了３次＋１０ＧＺ／３ｍｉｎ（两次间间隔３０ｍｉｎ）作用。分别于暴露后３０ｍｉｎ、６ｈ和２４ｈ处死大鼠取脑，提取总ＲＮＡ，用建立的反转录聚合酶链反应（ＲＴ－ＰＣＲ）定量检测方法检测大鼠脑组织ｃ－ｆｏｓ和ｃ－ｊｕｎｍＲＮＡ表达水平。结果＋ＧＺ重复暴露后３０ｍｉｎ大鼠脑组织ｃ－ｆｏｓ和ｃ－ｊｕｎｍＲＮＡ明显升高，分别是对照组的２．８倍和１．５倍，但６ｈ和２４ｈ时恢复正常。结论＋ＧＺ重复暴露可诱发大鼠脑组织ｃ－ｆｏｓ和ｃ－ｊｕｎｍＲＮＡ的表达，这种表达快速且短暂，在＋ＧＺ所致脑损害的病理过程中起一定作用。
Objective To investigate the changes of c-fos and c-jun gene expression in rat brain after + GZ repeated exposure and to explore the molecular mechanism of + GZ-induced brain damage and to provide experimental evidence for the protection of + GZ. Methods Twenty-four male Wistar rats were randomly divided into control group, GZ + GZ group after repeated exposure for 30min, 6h and 24h. The G value of the control group was + 1GZ, and the rats in the experimental group underwent 3 times + 10GZ / 3min in the animal centrifuge (the interval was 30min between two times). The rats were sacrificed at 30min, 6h and 24h after exposure, respectively. The total RNA was extracted and the expressions of c-fos and c-jun mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR) Level. Results The levels of c-fos and c-jun mRNA in brain tissue of rats exposed to + GZ for 30min were 2.8 and 1.5 times higher than that of the control group, respectively, but returned to normal after 6 and 24h. Conclusions + GZ repeated exposure can induce the expression of c-fos and c-jun mRNA in rat brain. The expression of c-fos and c-jun mRNA is rapid and transient, which play a role in the pathological process of brain damage induced by + GZ.