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用混合去垢剂增溶低离子强度条件下的温和电泳方法,对菠菜叶绿体类囊体膜和不同PSⅡ制剂进行了色素蛋白复合体组成的分析.并结合SDS聚丙烯酰胺凝胶电泳和吸收光谱对部分色素蛋白复合体进行了鉴定.此法简便、迅速、游离色素少.
The composition of the pigment protein complex of spinach chloroplast thylakoid membranes and different PS Ⅱ preparations was analyzed by the mild electrophoresis method with mixed detergents under low ionic strength. Some pigment protein complexes were identified by SDS polyacrylamide gel electrophoresis and absorption spectroscopy. This method is simple, rapid, less pigment free.