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目的探讨人参多糖体外抑制人非小细胞肺癌A549细胞的作用。方法应用MTT法检测药物对非小细胞肺癌A549细胞增殖的影响,应用流式细胞技术检测细胞周期变化,Hoechst 33258/PI双染色法观察细胞凋亡形态的变化。结果人参多糖在体外可抑制人非小细胞肺癌A549细胞增殖,促进细胞凋亡,阻滞细胞周期在G2/M期;荧光染色可见,随药物浓度增加细胞凋亡率增加,表现为染色质凝集、细胞核碎裂等典型的细胞凋亡形态变化。结论 人参多糖在体外可抑制人非小细胞肺癌A549细胞增殖,诱导细胞凋亡。
Objective To investigate the inhibitory effect of polysaccharide from ginseng on human non-small cell lung cancer A549 cells in vitro. Methods MTT assay was used to detect the proliferation of non-small cell lung cancer A549 cells. The cell cycle was detected by flow cytometry. Hoechst 33258 / PI double staining was used to observe the changes of apoptosis. Results Ginseng polysaccharide can inhibit proliferation and promote cell apoptosis of human non-small cell lung cancer A549 cells in vitro, and arrest the cell cycle in G2 / M phase. Fluorescent staining shows that apoptosis rate increases with the increase of drug concentration, showing chromatin condensation , Nuclear fragmentation and other typical morphological changes of apoptosis. Conclusion Ginseng polysaccharide can inhibit proliferation and induce apoptosis of human non-small cell lung cancer A549 cells in vitro.