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目的:考察低温空气等离子体对医用聚四氟乙烯(PTFE)表面大肠杆菌的灭活效果以及灭菌机理。方法:采用Langmuir双电子探针和电子自旋共振(ESR)诊断技术分别定量测定了远程低温空气等离子体场中各活性物种的分布。并通过扫描电镜(SEM)对灭菌后细胞破碎情况进行观察,细胞泄漏物质检测,反映腔温度变化和紫外线对灭菌影响分析其灭菌机理。结果:本实验条件下,空气等离子灭菌的最适宜操作参数为,放电功率100W,空气流量40sccm,放电时间120s。通过细胞形态学的观察和蛋白质泄漏量检测,结果证实等离子体处理后的大肠杆菌细胞肿胀、疏松,因此可以得出空气等离子体对大肠杆菌的灭菌机理为,电子、离子、自由基等活性粒子作用于细胞壁和细胞膜,使之肿胀、疏松、断裂,通透性增加,内容物流出是导致大肠杆菌死亡的直接原因。结论:空气等离子体灭菌效果受等离子体处理参数的影响。
Objective: To investigate the inactivation effect and sterilization mechanism of cryogenic air plasma on medical polytetrafluoroethylene (PTFE) surface Escherichia coli. METHODS: Langmuir dual electron probe and ESR diagnostic techniques were used to quantitatively determine the distribution of each active species in the remote low temperature air plasma field. Scanning electron microscopy (SEM) was used to observe the cell breakage after sterilization, the detection of cell leakage material, the change of cavity temperature and the effect of ultraviolet light on sterilization. Results: Under the experimental conditions, the most suitable operating parameters for air plasma sterilization were discharge power 100W, air flow rate 40sccm and discharge time 120s. Through the observation of cell morphology and the detection of protein leakage, the results confirmed that the plasma-treated Escherichia coli cells swelled and loosened. Therefore, it could be concluded that the sterilization mechanism of the air plasma against Escherichia coli was electron, ion, free radical and the like Particles act on the cell wall and cell membrane, so that swelling, loose, broken, increased permeability, the contents of the outflow is the direct cause of death caused by E. coli. Conclusion: The air plasma sterilization effect is affected by the plasma processing parameters.