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目的建立一种高灵敏度的液质联用方法,用于快速测定大鼠血浆中天麻素的浓度,并计算了天麻素在大鼠体内的药代动力学参数。方法血浆样品加乙腈沉淀蛋白,以乙腈-水(68:32)为流动相,用ApolloC18色谱柱(4.6mm×150mm,5μm)分离,采用电喷雾离子化源(ESI)三重四级杆串联质谱,以选择离子监测(SIM)方式进行检测。结果天麻素线性范围为:0.0222～2.22μg·mL-1,回归方程为:A=301733+2×107C,R2=0.9949(n=7);低、中、高3个质量浓度的平均回收率分别为:99.55%、99.10%、98.65%。结论该法快速、灵敏、专属,适用于测定生物样本中天麻素的血药浓度。
Objective To establish a high sensitivity LC / MS method for the rapid determination of gastrodin in rat plasma and calculate the pharmacokinetic parameters of gastrodin in rats. Methods Plasma samples were precipitated with acetonitrile, separated on Apollo C18 column (4.6 mm × 150 mm, 5 μm) using acetonitrile-water (68:32) as mobile phase and electrospray ionization source (ESI) triple quadrupole tandem mass spectrometry , To select the ion monitoring (SIM) mode for testing. Results The linear range of gastrodin was 0.0222-2.22μg · mL-1. The regression equation was A = 301733 + 2 × 107C, R2 = 0.9949 (n = 7). The average recoveries of low, Respectively: 99.55%, 99.10%, 98.65%. Conclusion The method is rapid, sensitive and specific and is suitable for the determination of blood concentration of gastrodin in biological samples.