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【目的】探究氮肥对毛竹林土壤硝化和反硝化作用的影响,并分析其与主要土壤因子的相关关系,为毛竹林的抚育管理及可持续经营提供理论依据。【方法】以不施氮肥毛竹林为对照,应用气压过程分离(BaPS)方法,分季节测定施氮肥条件下毛竹林土壤总硝化和反硝化速率。【结果】施氮肥条件下毛竹林土壤总硝化和反硝化速率从春季到冬季变化规律相似,均呈现先升高后降低趋势,施肥毛竹林土壤总硝化和反硝化速率均以7月最高,当月相比分别比未施肥毛竹林高27.50%和44.60%;最低值分别出现在1月和10月,当月相比分别比未施肥毛竹林高45.58%和402.56%。【结论】施氮肥可以促进毛竹林土壤硝化和反硝化作用,提高土壤硝态氮和微生物生物量氮含量,氮肥和季节对总硝化和反硝化速率存在显著交互作用,土壤温度、含水率与土壤总硝化、反硝化速率均呈显著正相关。
【Objective】 The objective of this study was to investigate the effects of nitrogen fertilizers on soil nitrification and denitrification in Moso bamboo forest and to analyze its correlation with soil factors to provide theoretical basis for tending and management of Phyllostachys pubescens forest and sustainable management. 【Method】 The total nitrogen and denitrification rates of Phyllostachys pubescens plantations under different N application rates were determined by the atmospheric pressure process separation (BaPS) method. 【Result】 The results showed that the total nitrification and denitrification rate of Moso bamboo plantation changed from spring to winter under the condition of nitrogen fertilizer, both of them increased firstly and then decreased. The total nitrification and denitrification rates of Moso bamboo plantation were the highest in July, Compared with the non-fertilized Moso bamboo forest, they were 27.50% and 44.60% higher than the non-fertilized Moso bamboo forest respectively. The lowest values appeared in January and October respectively, compared with 45.58% and 402.56% respectively higher than the non-fertilized Moso bamboo forest. 【Conclusion】 Nitrogen fertilizer can promote soil nitrification and denitrification in soil, increase soil nitrate content and microbial biomass nitrogen content. Nitrogen fertilizer and seasons have significant interaction on total nitrification and denitrification rate. Soil temperature, water content and soil Total nitrification and denitrification rates were significantly and positively correlated.