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在二维液相色谱中,第二维的分离速度是制约其发展的重要因素.升高色谱柱温度可以有效降低流动相粘度,加快溶质在两相间的传质速率,有效加快分析速度.以离子交换色谱法(WAX)为第一维分离模式和反相色谱法(RP)为第二维分离模式,十通阀和两个捕集柱为接口,通过将第二维色谱柱温度升高到80℃和提高流量到3 mL/min,构建了高温WAX/RP二维液相色谱系统.以4种标准蛋白的酶解物为样品评价系统的分离性能,第一维共有33个馏分被捕集并导入到第二维分析,高温二维液相色谱系统识别出187个色谱峰.
In 2D liquid chromatography, the separation speed of the second dimension is an important factor restricting its development.Improving the temperature of the column can effectively reduce the viscosity of the mobile phase, accelerate the mass transfer rate of the solute between the two phases, and effectively speed up the analysis. Ion Exchange Chromatography (WAX) for the first dimension separation mode and reverse phase chromatography (RP) for the second dimension separation mode, the ten-way valve and the two capture columns for the interface, by increasing the temperature of the second dimension column To 80 ℃ and increasing the flow rate to 3 mL / min, a high-temperature WAX / RP two-dimensional liquid chromatography system was constructed.The separation performance of the system was evaluated by using four standard protein hydrolysates as sample, a total of 33 fractions Captured and imported into the second dimension analysis, the High Temperature Two-Dimensional Liquid Chromatography System identified 187 peaks.