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本文应用高压液相 电化学 (HPLC EC)技术对 5 2名男性不育患者精子DNA中 8 羟基脱氧鸟苷 (8 OHdG)进行测定 ,同时检测精液量、精子密度、精子活率、正常形态精子率 ,及头部精子畸形等精液参数 ,并进行相关性分析。结果显示 ,当精子DNA中 8 OHdG超过 10 .5 0时 ,其头部畸形率明显增加。精子DNA中 8 OHdG与精液量 (r =- 0 .4 7,P <0 .0 0 1)、正常形态精子率 (r=- 0 .36 ,P <0 .0 1)成一定的负相关 ,且与头部畸形精子率成正相关 (r =0 .6 9,P <0 .0 0 1)。结果提示 ,精子DNA中 8 OHdG与精液质量存在一定的相关性 ,表明精液质量的下降与精子DNA的损伤存在一定联系 ,而内源及外源性活性氧是可能的原因之一 ,其可能的机制尚需进一步研究。
In this paper, 5-hydroxydeoxyguanosine (8-OHdG) in sperm DNA of 52 male infertile patients was determined by HPLC-EC with simultaneous detection of sperm quantity, sperm density, sperm motility, normal morphology Rate, and head sperm deformity and other semen parameters, and correlation analysis. The results showed that when the 8 OHdG in sperm DNA exceeded 10 .5 0, its head deformity rate increased significantly. 8 OHdG in sperm DNA was negatively correlated with the amount of semen (r = - 0.47, P <0.01) and normal morphology sperm (r = - 0.36, P <0.01) , And had a positive correlation with head abnormal sperm rate (r = 0.69, P <0.001). The results suggest that there is a correlation between 8-OHdG and sperm quality in sperm DNA, indicating that there is a certain relationship between sperm quality and sperm DNA damage. However, both endogenous and exogenous reactive oxygen species are possible reasons Mechanism needs further study.