论文部分内容阅读
目的观察小鼠胚胎干(ES)细胞植人大鼠隔区和海马内后的分化情况,并对其作研究和分析。方法成年SD大鼠35只,体质量200～250g,雌雄不限。以SD大鼠为宿主,将ES细胞移植入宿主隔区和海马内,移植后l,2,3,4和8周后取脑,冷冻切片,进行Nissl,M6,NSE,GFAP免疫组织化学染色。结果ES细胞移植入大鼠隔区和海马内后,从第2周开始表达M6、GFAP、NSE等抗原,持续至第4周,主要位于移植区内,较少迁移。结论小鼠ES细胞移植入大鼠隔区和海马内后,分化为神经元,神经胶质细胞。
Objective To observe the differentiation of mouse embryonic stem (ES) cells into rat compartment and hippocampus and to study and analyze them. Methods Thirty-five adult SD rats weighing 200-250g were randomly divided into male and female. ES cells were transplanted into the host compartment and the hippocampus with SD rats as host. After 1, 2, 3, 4 and 8 weeks after transplantation, the brain and frozen sections were taken for Nissl, M6, NSE and GFAP immunohistochemical staining . Results After transplanted into the isolated rat septum and hippocampus, ES cells expressed antigens such as M6, GFAP and NSE from the 2nd week until the 4th week, mainly in the transplantation area with less migration. Conclusions Mouse ES cells are differentiated into neurons and glial cells after transplanted into the compartments of rats and hippocampus.