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本文改进和发展了一种能测定大气降水中的微量H_2O_2的分光光度法。该法采用酶催化反应,有较好的选择性和较高的灵敏度。将原缓冲体系HAc-NaAc改为KHP后,克服了HAc-NaAc体系的缺点并大大简化了测定步骤。由工作曲线计算所得的摩尔吸收系数为7.4×10~4L/(cm·mol),最低可测浓度为0.3μmol/l.Mn~(2+)对测定有轻度干扰,有机氢过氧化物和NO_(2-)有较严重的干扰,但这些物质在降水中的浓度很低,总于挑一般小于5％,且通过加入过氧化氢酶的办法可以排除。对照结果表明,本法与国际公认的POPHA荧光法有很好的可比性。
This paper improves and develops a spectrophotometric method for the determination of trace H 2 O 2 in precipitation. The method uses enzyme-catalyzed reaction, has better selectivity and higher sensitivity. After changing the original buffer system HAc-NaAc to KHP, the disadvantages of the HAc-NaAc system were overcome and the measurement procedure was greatly simplified. The molar absorption coefficient calculated from the working curve is 7.4 × 10 ~ 4L / (cm · mol), the lowest measurable concentration is 0.3μmol / 1.Mn ~ (2+) has a slight interference with the determination. The organic hydroperoxide And NO_ (2-) are more serious interference, but the concentration of these substances in precipitation is very low, the total pick is generally less than 5%, and through the addition of catalase can be ruled out. The control results show that this method is well comparable with the internationally accepted POPHA fluorescence method.