The patterns of soil nitrogen(N) isotope composition at large spatial and temporal scales and their relationships to environmental factors illustrate N cycle and sources of N,and are integrative indicators of the terrestrial N cycle and its response to global change. The objectives of this study were:i) to investigate the patterns of soil N content and natural abundance of 15N(δ15N) values in different ecosystem types and soil profiles on the Qinghai-Tibetan Plateau; ii) to examine the effects of climatic factors and soil characteristics on the patterns of soil N content and soil δ15N values; and iii) to test the relationship between soil δ15N values and soil C/N ratios across ecosystems and soil profiles. Soil profiles were sampled at 51 sites along two transects 1 875 km in length and 200 km apart and distributed in forest,meadow and steppe on the Qinghai-Tibetan Plateau. Each site was sampled every 10 cm from a soil depth of 0 to 40 cm and each sample was analyzed for soil N content and δ15N values. Our results indicated that soil N and δ15N values(0–40 cm) in meadows were much higher than in desert steppe. Soil N decreased with soil depth for each ecosystem,while variations of soil δ15N values along soil profiles were not statistically significant among most ecosystems but for mountain meadow,lowland meadow,and temperate steppe where soil δ15N values tended to increase with soil depth. The parabolic relationship between soil δ15N values and mean annual precipitation indicated that soil δ15N values increased with increasing precipitation in desert steppe up to 500 mm,and then decreased with increasing precipitation across all other ecosystems. Moreover,the parabolic relationship between δ15N values and mean annual temperature existed in all individual ecosystem types. Soil N and δ15N values(0–40 cm) increased with an increase in soil silt and clay contents. Furthermore,a threshold of C/N ratio of about 11 divided the parabolic relationship between soil δ15N values and soil C/N ratios into positive(C/N < 11) and negative(C/N > 11) parts,which was valid across all ecosystems and soil profiles. The large explanatory power of soil C/N ratios for soil δ15N values suggested that C and N concentrations,being strongly controlled by precipitation and temperature,were the primary factors determining patterns of soil δ15N on the Qinghai-Tibetan Plateau. The patterns of soil nitrogen (N) isotope composition at large spatial and temporal scales and their relationships to environmental factors illustrate N cycle and sources of N, and are integrative indicators of the terrestrial N cycle and its response to global change. The objectives of this i) to investigate the patterns of soil N content and natural abundance of 15N (δ15N) values ​​in different ecosystem types and soil profiles on the Qinghai-Tibetan Plateau; ii) to examine the effects of climatic factors and soil characteristics on the patterns of soil N content and soil δ15N values; and iii) to test the relationship between soil δ15N values ​​and soil C / N ratios across ecosystems and soil profiles. Soil profiles were sampled at 51 sites along two transects 1 875 km in length and 200 km apart and distributed in forest, meadow and steppe on the Qinghai-Tibetan Plateau. Each site was sampled from 10 cm from a soil depth of 0 to 40 cm and each sample was analyzed for soil N cont ent and δ15N values. Our results indicated that soil N and δ15N values ​​(0-40 cm) in meadows were much higher than in desert steppe. Soil N minus with soil depth for each ecosystem, while variations of soil δ15N values ​​along soil profiles were not well significant among most ecosystems but for mountain meadow, lowland meadow, and temperate steppe where soil δ15N values ​​tended to increase with soil depth. The parabolic relationship between soil δ15N values ​​and mean annual precipitation indicates that soil δ15N values ​​increased with increasing precipitation in desert Moreover, the parabolic relationship between δ15N values ​​and mean annual temperature existed in all individual ecosystems. Soil N and δ15N values ​​(0-40 cm) increased with an increase in soil silt and clay contents. Furthermore, a threshold of C / N ratio of about 11 divided the parabolic relationship between soil δ15N values and soil C / N ratios into positive (C / N <11) and negative (C / N> 11) parts, which was valid across all ecosystems and soil profiles. The large explanatory power of soil C / N ratios for soil δ15N values ​​suggested that C and N concentrations, being strongly controlled by precipitation and temperature, were the primary factors determining patterns of soil δ15N on the Qinghai-Tibetan Plateau.