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应用SDS-PAGE技术分析了陕优225等16个品种及3个组合杂种后代分离材料高分子量谷蛋白(HMWg)亚基组成及与面包品质的关系。结果表明,普通小麦中罕见的Glu-B_1编码亚基14+15象Glu-D_1编码亚基5+10一样,对面包品质有重要贡献,存在于陕优225、小偃6号等优质品种中。国内大3数品种Glu-1品质得分与SDS沉淀值显著正相关,HMwg亚基组成遗传F_1呈共显性,F_2、F_3异质位点呈亲本1:混合型:亲本2=1:2:1分离比例。F_1沉淀值呈中亲偏低值,F_3品系沉淀值为:HMWg亚基组成优质纯合型>混合型>劣质纯合型。
SDS-PAGE was used to analyze the composition of high molecular weight glutenin (HMWg) subunit of 16 hybrids of Shanyou 225 and three hybrids offspring, and their relationship with bread quality. The results showed that the common Glu-B 1 coding subunit 14 + 15, like Glu-D 1 coding subunit 5 + 10, had an important contribution to bread quality and existed in high quality varieties such as Shanyou 225 and Xiaoyan 6. The Glu-1 quality score of domestic large three cultivars was positively correlated with SDS sedimentation value. The genetic F 1 of HMWg subunit was co-dominant, the F 2 and F 3 heterozygous loci were the parents 1: mixed type: parent 2 = 1: 2: 1 separation ratio. F_1 sedimentation value was moderately low value, F_3 strain sedimentation value: HMWg subunit composition of high quality homozygous> mixed> poor homozygous.