目的利用Vero细胞微载体技术规模化培养轮状病毒。方法利用5 L生物反应器进行Vero细胞的微载体培养,待细胞密度达1.5×106个/ml时,以0.05 MOI接种轮状病毒P[2]G3株,于37℃连续培养6 d后收获病毒,期间每天取样观察细胞病变(CPE),并检测病毒感染性滴度。结果在轮状病毒规模化培养的初期,其病毒滴度逐渐升高,至48 h达到最高。在上清中,病毒的滴度为5.5CCID50/ml;在细胞裂解产物中,病毒的滴度为3.75CCID50/ml。结论Vero细胞微载体规模化培养轮状病毒可提高病毒的滴度。 Objective To develop rotavirus on a large scale by using Vero cell microcarrier technology. Methods Vero cells were cultured in microcarriers using 5 L bioreactor. When the cell density reached 1.5 × 106 cells / ml, rotavirus P [2] G3 strain was inoculated with 0.05 MOI and harvested continuously at 37 ℃ for 6 days The virus was sampled daily for cytopathic effect (CPE) and viral infectivity was tested. Results At the initial stage of large-scale rotavirus culture, the virus titer gradually increased and reached the highest level at 48 hours. In the supernatant, the virus titer was 5.5 CCID50 / ml; in the cell lysate, the virus titer was 3.75 CCID50 / ml. Conclusion Vero cell micro-carrier scale rotavirus can increase the virus titer.