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目的 :了解大肠癌患者明胶酶 A基因表达的情况。方法 :50例大肠癌患者术中取癌组织及正常组织 ;提取组织总 RNA;用反转录共扩增定量 PCR方法测定其明胶酶 A基因表达水平。结果 :大肠癌组织明胶酶 A( MMP2 )的基因表达明显高于正常大肠组织 ;发生局部淋巴结转移者组织明胶酶 A基因表达水平明显高于局部淋巴结无转移者。结论 :明胶酶 A在大肠癌的浸润、转移中可能起重要作用。
Objective: To understand the expression of gelatinase A gene in patients with colorectal cancer. Methods: Fifty patients with colorectal cancer underwent surgery to take cancer tissues and normal tissues. Total RNA was extracted from the tissues. The expression of gelatinase A gene was determined by reverse transcription-polymerase chain reaction (RT-PCR). Results: The gene expression of gelatinase A (MMP2) in colorectal cancer tissues was significantly higher than that in normal colorectal tissues. The expression of gelatinase A gene in local lymph node metastasis was significantly higher than that in local lymph nodes without metastasis. Conclusion: Gelatinase A may play an important role in the invasion and metastasis of colorectal cancer.