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目的分析浙江省宁波市2003-2014年肠炎沙门菌脉冲场凝胶电泳(PFGE)分型及耐药特征。方法采用Pulse Net China网络实验室的标准方法,对2003-2014年间分离的68株肠炎沙门菌进行XbaⅠ和BlnⅠ酶切分型,使用Bio Numerics软件对菌株的PFGE图谱进行聚类分析;使用纸片扩散法检测菌株对10种抗生素的耐药性。结果 68株肠炎沙门菌经XbaⅠ酶切分为8个PFGE带型。其中JEGX01.NB0001型为最优势型别,共53株,带型相似度90%以上的菌株占95.6%(65/68),2003年和2013年超过80例以上的食源性暴发案例的肠炎菌株经双酶切聚类证实存在100%的同源性。所有肠炎沙门菌对抗生素耐药率为氨苄西林23.5%、强力霉素4.4%、氨曲南、头孢吡肟和头孢噻肟均为2.9%、氯霉素和复方新诺明均为1.5%,对庆大霉素、环丙沙星、亚胺培南均敏感,发现2株多重耐药菌。结论宁波市散发和暴发的肠炎沙门菌分离株之间的分子带型差异度小,引发食源性暴发的流行株克隆之间存在流行病学关联。目前肠炎沙门菌对抗生素的耐药率处于较低水平,仍需加强对多重耐药株的监控。
Objective To analyze the typing and drug resistance of Salmonella enteritidis pulsed-field gel electrophoresis (PFGE) from 2003 to 2014 in Ningbo, Zhejiang Province. Methods Sixty isolates of Enterobacter enteritis isolated from 2003 to 2014 were genotyped by XbaⅠand BlnⅠ using the standard method of Pulse Net China Network Laboratory. The PFGE patterns of the isolates were analyzed by Bio Numerics software. Drug resistance of 10 strains of antibiotics tested by diffusion method. Results Sixty enterococci enteritidis were divided into 8 PFGE bands by Xba Ⅰ digestion. Among them, JEGX01.NB0001 was the most predominant type, with a total of 53 isolates, 95.6% (65/68) strains with more than 90% banding similarity, more than 80 cases of foodborne outbreaks of enteritis in 2003 and 2013 Strain by double enzyme digestion cluster confirmed 100% homology. All S. Enteritidis antibiotic resistance rates of ampicillin 23.5%, doxycycline 4.4%, aztreonam, cefepime and cefotaxime were 2.9%, chloramphenicol and cotrimoxazole 1.5% Gentamicin, ciprofloxacin, imipenem are sensitive and found 2 multi-resistant bacteria. Conclusions There is a small difference in molecular band between Salmonella enteritidis isolates in Ningbo City and outbreaks of Epidemiological epidemiology. Salmonella enteritidis antibiotic resistance rates are currently at a low level, the need to strengthen the monitoring of multiple drug-resistant strains.