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目的探讨黄体生成素受体(LHR)和血管内皮生长因子(VEGF)在围着床期小鼠卵巢中的生物学作用。方法应用免疫组织化学SABC法和图像分析,检测LHR与VEGF在昆明小鼠(n=28)动情期、孕1d、孕4d和孕6d卵巢中的分布状况和变化规律。结果LHR与VEGF免疫阳性细胞具有相似的分布和变化规律。在动情期,较大的卵泡周围的基质细胞呈强阳性着色,与其他组相比,差异显著(P<0.05)。随着妊娠的继续,颗粒黄体细胞的着色逐渐增强,在孕6d时表达最强。在动情期和孕1d,部分血管内皮和血细胞分别呈VEGF和LHR强阳性着色。从孕1d开始,卵泡膜细胞呈LHR阳性着色。结论LHR和VEGF的表达与围着床期小鼠卵巢中卵泡的发育、排卵和黄体形成过程密切相关。
Objective To investigate the biological effects of luteinizing hormone receptor (LHR) and vascular endothelial growth factor (VEGF) in peri-implantation mouse ovary. Methods Immunohistochemical SABC method and image analysis were used to detect the distribution and changes of LHR and VEGF in ovary of Kunming mice (n = 28) during estrus, pregnancy 1d, pregnancy 4d and pregnancy 6d. Results LHR and VEGF immunoreactive cells had similar distribution and variation. During the estrus period, stromal cells around the larger follicles showed strong positive staining compared with other groups (P <0.05). With the continuation of pregnancy, the staining of granular luteal cells gradually increased, the expression of the strongest in pregnancy 6d. In estrus and pregnancy 1d, some of the vascular endothelial cells and blood cells were strongly positive for VEGF and LHR staining. From the first day of pregnancy, the antral follicular cells showed LHR staining. Conclusion The expressions of LHR and VEGF are closely related to the ovarian follicle development, ovulation and luteal formation in the perinatal mouse.