利用中性蛋白酶对油茶籽蛋白进行酶解的工艺研究。以pH、酶解温度、酶解时间、加酶量、底物浓度为单因素进行了单因素酶解研究,再在单因素基础上以水解度为响应值对酶解条件进行响应面分析,得到最佳工艺条件为:酶解温度53.5℃、pH 7、加酶量4 364.0 U/g底物、酶解时间4 h,底物浓度2.9%。 Study on the enzymatic hydrolysis of Camellia oleifera by neutral protease. One-factor enzymolysis was carried out with pH, ​​temperature, time, amount of enzyme and substrate concentration as the single factor, and then the reaction surface was analyzed by the degree of hydrolysis as the response value on the basis of single factor. The optimum conditions were as follows: temperature 53.5 ℃, pH 7, enzyme loading 4 364.0 U / g substrate, hydrolysis time 4 h, substrate concentration 2.9%.