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目的探讨肿瘤相关基因RAN多态性与广西人群乙型肝炎病毒(HBV)相关肝细胞癌(HCC)遗传易感性的相关性。方法选取位于RAN启动子区rs7132224和3’非翻译区rs14035两个单核苷酸多态性(SNP)为遗传标记;采用SNPstream对340例HBV相关的HCC和361例对照个体进行基因分型;Logistic回归分析计算OR和95%CI。结果①rs7132224、rs14035和HBV相关HCC遗传易感性相关性评价结果分别为OR=1.43,95%CI=1.04～1.98,P=0.02;OR=1.43,95%CI=0.57～4.11,P=0.40;②对人群进行性别、年龄、吸烟、饮酒状态、一级生物学亲属HCC家族史分层分析,发现rs7132224、rs14035和各亚人群HBV HCC发生均无明显相关性。结论 rs7132224和HBV相关HCC遗传易感性显著相关,而rs14035与HBV相关HCC遗传易感性无显著相关性。rs7132224、rs14035与性别、年龄、饮酒状态、吸烟状态、一级生物学亲属HCC家族史在HBV相关HCC发生风险中无交互作用。
Objective To investigate the association between RAN polymorphism of tumor associated gene and genetic predisposition of hepatocellular carcinoma (HCC) associated with hepatitis B virus in Guangxi population. Methods Two single nucleotide polymorphisms (SNPs), rs7132224 in RAN promoter region and rs14035 in 3 ’untranslated region, were selected as genetic markers. 340 HBV-associated HCCs and 361 control individuals were genotyped by SNPstream. Logistic regression analysis calculated OR and 95% CI. Results ① The correlation of rs7132224, rs14035 and HBV-related HCC genetic susceptibility was OR = 1.43, 95% CI = 1.04-1.98, P = 0.02; OR = 1.43, 95% CI = 0.57-4.11, P = 0.40; There was no significant correlation between rs7132224, rs14035 and HBV subtype HCC in all sub-populations according to gender, age, smoking, drinking status and family history of HCC in first-degree biological relatives. Conclusion There is a significant correlation between rs7132224 and genetic susceptibility of HBV-related HCC, but there is no significant correlation between rs14035 and genetic predisposition of HBV-related HCC. rs7132224, rs14035 had no interaction with the HCC family history of HBV-related HCC in terms of gender, age, drinking status and smoking status.