本文应用高效液相色谱等手段从人胎肝中纯化肝再生刺激物质(HSS),并应用多种生物学技术证明HSS可剂量相关性地促进肝细胞DNA合成,每毫升培养液中含38ng的HSS即可促进肝细胞DNA合成增加2.5倍;且与胰岛素、表皮生长因子有协同作用。提纯后的HSS杂蛋白含量降低6 000倍,特异活性提高840倍;SDS-PAGE银染色仅显示一条蛋白区带,分子量为12.8kd,并对其氨基酸组成与比例进行了分析。 This article uses high performance liquid chromatography and other means to purify liver regeneration stimulatory substances (HSS) from human fetal liver, and applies a variety of biological techniques to prove that HSS can promote DNA synthesis of hepatocytes dose-dependently, containing 38 ng per mL of culture fluid. HSS can promote 2.5-fold increase in DNA synthesis in hepatocytes; and synergistic effects with insulin and epidermal growth factor. The purified HSS protein content decreased by 6,000-fold, and the specific activity increased 840-fold. SDS-PAGE silver staining showed only one protein band with a molecular weight of 12.8 kd, and its amino acid composition and ratio were analyzed.