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用~3H—TdR释放法测定C_(57)BL/6小鼠脾细胞对YAC-1细胞林的NK活性,首次将该法应用于研究NK细胞对γ线的辐射敏感性,得出如下结论:(1)小鼠整体照射后24h脾脏NK活性的D_(37)约为17Gy,D_0约为11Gy;在24～32Gy范围内仍保留着30%的NK活性,存活曲线为反“S”形,验证了NK细胞总体对辐射的相对耐受性。(2)脾细胞离体或整体照射1～2Gy后即刻NK活性均迅速下降,在2～24Gy内,离体和整体照射组的NK活性分别维持在40%～80%和50%,前者高于后者,说明NK细胞对离体照射比对整体照射更不敏感。(3)小鼠整体照射后24h,全脾细胞数在较小剂量(<4Gy)内急剧下降,然后随剂量增大变化平缓。可以推测NK活性的变化与脾细胞总数的改变有一定关系。(4)综合分析本实验结果,可以设想NK细胞本身可能存在着辐射敏感性不同的亚群。(5)用~3H—TdR释放法测定NK细胞的辐射敏感性,自然释放率低,重复性好,可靠易行。
The ~3H-TdR release method was used to determine the NK activity of C57BL/6 mouse spleen cells on YAC-1 cell line. The method was first applied to study the gamma radiation sensitivity of NK cells, and the following conclusions were drawn: : (1) The NK activity of spleen NK activity was about 17 Gy and D_0 was about 11 Gy at 24 h after irradiation. In the 24-32 Gy range, 30% of NK activity was still retained, and the survival curve was anti-“S” shaped. This verifies the relative tolerance of NK cells to radiation. (2) Immediately after ex vivo or whole body irradiation of splenocytes for 1-2 Gy, the NK activity rapidly decreased. Within 2-24 Gy, the NK activity in the isolated and whole irradiated groups maintained at 40%-80% and 50% respectively. The former was high. In the latter case, it was demonstrated that NK cells are less sensitive to ex vivo irradiation than to overall irradiation. (3) The number of whole spleen cells dropped sharply within a small dose (<4 Gy) 24 h after irradiation, and then changed gradually with increasing dose. It can be speculated that changes in NK activity have a relationship with changes in the total number of spleen cells. (4) A comprehensive analysis of the experimental results suggests that NK cells may have subpopulations with different radiation sensitivities. (5) The radiosensitivity of NK cells was measured by ~3H-TdR release method. The natural release rate was low, and the repeatability was good and reliable.