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目的 观察大鼠ICC介导促胰液素抑制胃平滑肌细胞收缩作用。方法 采用大鼠胃体上 1 3起博区及胃窦环行肌 8× 2mm肌条在 95 %O2 ,5 %CO2 的恒温 37℃灌流Kreb s液 ,通过张力换能器输入生理记录仪记录胃肌条的机械运动。用美蓝 (methyleneblue)加光照破坏ICC ,观察促胰液素作用
Objective To observe the inhibitory effect of ICC on the contraction of gastric smooth muscle cells induced by ICC in rats. Methods Krebs fluid was perfused by 95% O2 and 5% CO2 at a constant temperature of 37 ° C on a strip of 8 × 2 mm muscle strips of gastric antrum and 13 rabbits in the gastric corpus. Tensiometer was used to record the gastric Mechanical muscle strips. Use Methyleneblue plus light to destroy ICC and observe the effect of secretin