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目的探讨粒／巨噬细胞集落刺激因子（ＧＭ－ＣＳＦ）及白介素－４（ＩＬ－４）对正常成人及大肠癌患者树突状细胞（ＤＣ）表面人白细胞抗原（ＨＬＡ）－ＤＲ及Ｂ７等免疫分子表达及其免疫功能的影响。方法分离正常成人（ｎ＝１０）及大肠癌患者（ｎ＝１０）外周血ＤＣ，以ＧＭ－ＣＳＦ及ＩＬ－４联合刺激正常成人及大肠癌者ＤＣ，检测ＧＭ－ＣＳＦ及ＩＬ－４联合刺激前后ＤＣ表面ＨＬＡ－ＤＲ及Ｂ７表达水平及ＤＣ免疫功能变化。结果经ＧＭ－ＣＳＦ及ＩＬ－４联合刺激后，大肠癌患者ＤＣ表面ＨＬＡ－ＤＲ及Ｂ７表达水平由９．２±１．４、８．６±１．５增至１７．２±１．２、１５±１．３ＶＯＦ（Ｐ＜０．０１），ＤＣ免疫诱导能力由６３５６±１１２增至１０４２±１４６ｃｐｍ（Ｐ＜０．０１）；正常成人ＤＣ表面ＨＬＡ－ＤＲ及Ｂ７表达水平由１５±１．６、１６±０．９增至１８±１．５、１８±１．３ＶＯＦ（Ｐ＞０．０５），ＤＣ免疫诱导能力由９８０６±１１３增至１１０８±１２７ｃｐｍ（Ｐ＞０．０５）。结论ＧＭ－ＣＳＦ及ＩＬ－４联合刺激能增强正常成人及大肠癌患者ＤＣ表面ＨＬＡ－ＤＲ及Ｂ７表达水平并进一步增强ＤＣ免疫功能。提示联合应用ＧＭ－ＣＳＦ及ＩＬ－４可能成为提高宿主抗?
Objective To investigate the effects of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) on the surface of dendritic cells (DCs) in normal adult and colorectal cancer patients. Human leukocyte antigen (HLA)-DR and B7, etc. The expression of immune molecules and the impact of their immune function. Methods Peripheral blood DC was isolated from normal adults (n=10) and colorectal cancer patients (n=10). Normal and adult colorectal DCs were stimulated with GM-CSF and IL-4 to detect the combined stimulation of GM-CSF and IL-4. Changes of HLA-DR and B7 expression and DC immune function in DC surface before and after. Results After combined stimulation of GM-CSF and IL-4, the expression levels of HLA-DR and B7 on DC surface of colorectal cancer patients increased from 9.2±1.4, 8.6±1.5 to 17.2±1.2. At 15±1.3 VOF (P<0.01), the immune induction ability of DC increased from 6356±112 to 1042±146 cpm (P<0.01); the level of HLA-DR and B7 expression on normal adult DC surface was 15±1. The levels of .6 and 16±0.9 increased to 18±1.5, 18±1.3 VOF (P>0.05), and the immune induction ability of DC increased from 9806±113 to 1108±127 cpm (P>0.05). Conclusion The combined stimulation of GM-CSF and IL-4 can enhance the expression of HLA-DR and B7 on the surface of normal adult and colorectal cancer DCs, and further enhance the DC immune function. It suggests that the combined use of GM-CSF and IL-4 may be used to increase host resistance.