实验用HRP逆行追踪法和免疫细胞化学ABC法,研究了大鼠中缝背核内5-羟色胺、P物质以及亮脑啡肽三种传入纤维的脑干起源。发现中央上核,中缝大核,中脑导水管周围灰质,脚间核和第Ⅳ脑室中央灰质均有数量不等的5-羟色胺、P物质和亮脑啡肽能神经元向中缝背核投射。结果提示:中缝背核接受脑干许多核团不同性质神经元的调控。 Experiments using HRP retrograde tracing and immunocytochemical ABC method to study the rat dorsal raphe nucleus 5-hydroxytryptamine, substance P, and bright enkephalin three afferent fibers of the stem origin. Found that the central superior nucleus, the stapes of the middle stapes, the midbrain periaqueductal gray, the intercostal nucleus, and the fourth ventricle central gray matter all have varying amounts of serotonin, substance P, and enkephalinine neurons projecting toward the dorsal raphe . The results suggest that the dorsal raphe nuclei accept the regulation of many different types of neurons in the brain stem.