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目的:探讨血清VEGF、bFGF浓度在评价骨肉瘤患者的预后及其肿瘤转移复发中的价值。方法:采用双抗体夹心ELISA法检测52例骨肉瘤患者术前、术后和60例健康体检者血清血管内皮生成因子(VEGF)、碱性成纤维因子(bF-GF)表达水平,并分析其与骨肉瘤患者术后肿瘤转移复发的相关性。结果:52例骨肉瘤患者术前血清VEGF、bFGF表达水平明显高于健康体检者(P<0.01),术后血清VEGF、bFGF表达水平与术前相比均明显下降(P<0.01),但仍高于健康体检者(P<0.01)。术前血清VEGF、bFGF的表达水平分别随原发肿瘤直径、Enneking分期、组织分化差而增高,具有统计学意义(P<0.05)。复发转移组和未复发转移组术前、术后血清VEGF、bFGF表达水平比较,差异存在显著性(P<0.01);经Cox回归多因素分析表明术前和术后血清VEGF、bFGF表达水平是影响患者术后复发转移的独立因素之一(P<0.05)。结论:术前和术后检测骨肉瘤患者血清VEGF、bFGF的表达水平可能对客观预测肿瘤术后转移复发具有一定的参考价值。
Objective: To investigate the value of serum VEGF and bFGF in the evaluation of the prognosis of patients with osteosarcoma and its tumor metastasis and recurrence. Methods: Serum levels of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bF-GF) in 52 cases of osteosarcoma were detected by double antibody sandwich ELISA. Correlation with postoperative tumor metastasis and recurrence in osteosarcoma patients. Results: The serum levels of VEGF and bFGF in 52 patients with osteosarcoma were significantly higher than those in healthy subjects (P <0.01). The levels of VEGF and bFGF in serum after operation were significantly lower than those before operation (P <0.01) Still higher than healthy people (P <0.01). Preoperative serum VEGF, bFGF expression levels were increased with the diameter of the primary tumor, Enneking staging, poor tissue differentiation, with statistical significance (P <0.05). The levels of VEGF and bFGF in serum of patients with recurrence and without recurrence were significantly higher than those without recurrence (P <0.01). The multivariate analysis by Cox regression showed that the levels of VEGF and bFGF in preoperative and postoperative serum were One of the independent factors affecting postoperative recurrence and metastasis (P <0.05). Conclusion: The expression of VEGF and bFGF in preoperative and postoperative detection of osteosarcoma may be useful for the objective prediction of postoperative metastasis and recurrence.