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对蚕豆萎蔫病毒2(BBWV2)分离物B935 RNA2全序列进行了测定,全长由3601个核苷酸组成(不包括3′端Poly(A))。RNA2包含一个长的开读框,该开读框起始于231位,终止于3428位,编码一个分子量为119 ku的多聚蛋白。对外壳蛋白亚基N端氨基酸序列测定表明外壳蛋白大亚基(LCP)和小亚基(SCP)是由119 ku多聚蛋白中谷酰胺与甘氨酸及谷酰胺与丙氨酸之间的切割产生的,LCP含有402个氨基酸,SCP含有197个氨基酸。与蚕豆病毒属(Fabavirus)病毒基因组核苷酸及编码的蛋白质氨基酸序列比较表明,B935与BBWV2分离物及广藿香轻花叶病毒具有很高的同源性,而与BBWV1分离物的同源性较低。B935与豇豆花叶病毒属(Comovirus)和线虫传多面体病毒属(Nepovirus)的基因组结构相似,但序列同源性很低。B935与豇豆病毒属病毒的LCP和SCP氨基酸具有共同的保守序列。用4株单克隆抗体进行TAS-ELISA测定表明B935与BBWV1分离物(NRS和PH3)抗原上有差异。
The complete sequence of B935 RNA2 from the isolate BBV2 of BBWV2 was sequenced. The full length cDNA consisted of 3601 nucleotides (excluding 3 ’Poly (A)). RNA2 contains a long open reading frame that begins at position 231 and ends at position 3428 encoding a polyprotein with a molecular weight of 119 ku. The amino acid sequence analysis of the N-terminal of the coat protein subunit showed that the LCP and the small subunit (SCP) of the coat protein are produced by the cleavage of glutamine and glycine and glutamine and alanine in the 119 ku polyprotein , LCP contains 402 amino acids and SCP contains 197 amino acids. Comparison with the nucleotide sequence of the Fabavirus virus genome and the encoded protein showed that B935 shared high homology with BBWV2 isolate and patchouli light mosaic virus, Sex is lower. B935 is similar to the genomic structure of Comovirus and Nepovirus but has low sequence homology. B935 share common conserved sequences with the LCP and SCP amino acids of cowpea virus. TAS-ELISA assay with four monoclonal antibodies showed differences between the B935 and BBWV1 isolates (NRS and PH3) antigens.