目的：对阿托品、东莨菪碱和樟柳碱等药物对映体的手性分离进行研究。方法：用高效毛细管电泳法（ＨＰＣＥ），分别以β环糊精（βＣＤ）和羧甲基β环糊精（ＣＭβＣＤ）为手性选择剂。结果：ＣＭβＣＤ可使阿托品和东莨菪碱的对映体得到部分分离，使樟柳碱的对映体得到完全分离；βＣＤ未能拆分阿托品和东莨菪碱的对映体，仅使樟柳碱的对映体得到部分分离。结论：ＣＭβＣＤ比βＣＤ对上述药物对映体具有更强的手性识别能力 OBJECTIVE: To study the chiral separation of enantiomers of atropine, scopolamine and anisodine. Methods: High performance capillary electrophoresis (HPCE) was used as chiral selector for βcyclodextrin (βCD) and carboxymethylβcyclodextrin (CMβCD) respectively. Results: CM β-CD can make the enantiomers of atropine and scopolamine partially separated so that the anisodine completely separated enantiomers; β CD failed to split enantiomers of scopolamine and scopolamine, only camphor The enantiomer of the beryl base is partially separated. Conclusion: CM β-CD than β CD on the drug enantiomer has a stronger chiral recognition ability