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用Affymetrix小麦基因芯片检测小麦成熟胚脱分化过程中CDPKs基因的表达变化的结果表明,在检测到的16个CDPKs基因中,有15个基因在小麦成熟胚脱分化过程中发生了有意义变化,其中上调表达基因有12个,上调2～7.5倍,下调表达基因有2个,下调3～6.1倍,在不同时点既有上调又有下调的1个。根据已知CDPKs生物功能和它们表达变化趋势以及这些变化趋势与脱分化过程中的吻合程度分析结果,推测CPK2A、CPK2B、CPK6和Os12g0169800基因参与了小麦成熟胚脱分化过程的启动和愈伤组织的形成。
The results of using the Affymetrix Wheat Gene Chip to detect the expression of CDPKs gene during the dedifferentiation of wheat mature embryos showed that 15 of the 16 CDPKs detected in the wheat dendifferentiation process had a significant change, Among them, 12 genes were up-regulated, 2 to 7.5 times up-regulated, 2 down-regulated genes were down-regulated by 3 to 6.1 times, and one was up-regulated and down-regulated at different time points. According to the known biological function of CDPKs and their expression trends and the relationship between these changes and dendritic differentiation, it was speculated that the CPK2A, CPK2B, CPK6 and Os12g0169800 genes involved in the initiation of dedifferentiation of wheat mature embryos and callus form.