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目的:筛选食管癌广泛高表达蛋白COX-2的HLA-A2/A3限制性广谱CTL表位。方法:运用生物信息学的方法,设计出P66(COX-266-74FLKPTPNTV)和P479(COX-2479-487ALYGDIDAV)两条新的抗原肽。Fmoc固相合成法合成抗原肽,对其与HLA-A2.1和HLA-A3分子的结合力和稳定性进行分析,以MTT实验检测其对三种肿瘤细胞的杀伤作用。结果P479对HLA-A2.1及HLA-A3均有较高的结合力,而P66结合力较低。P479对COX-2+的EC-9706、EC-1的表现出明显的诱导杀伤作用,而P66没有表现出明显的作随着肿瘤分子免疫学与分子生物学研究
OBJECTIVE: To screen HLA-A2 / A3-restricted broad-spectrum CTL epitopes of COX-2, a protein widely expressed in esophageal cancer. METHODS: Two new antigenic peptides, P66 (COX-266-74FLKPTPNTV) and P479 (COX-2479-487ALYGDIDAV), were designed using bioinformatics methods. Fmoc solid phase synthesis method for the synthesis of antigen peptide, its binding to HLA-A2.1 and HLA-A3 molecules and stability were analyzed by MTT assay to detect the killing effect of three kinds of tumor cells. Results P479 had higher binding to HLA-A2.1 and HLA-A3, while P66 had lower binding. P479 showed a significant inductive killing effect on COX-2 + EC-9706 and EC-1, while P66 did not show obvious effect. With the study of tumor molecular immunology and molecular biology