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目的:探讨人胚胎小肠嗜铬素A免疫反应(Chromogranin A-immunoreactive,CgA-IR)细胞的发育规律。方法:应用免疫组化技术和体视学方法,对40例6至38周的人胚胎小肠CgA-IR 细胞进行体视学定量分析。结果:人胚胎小肠CgA-IR细胞于人胚第7周出现,十二指肠最多,空肠次之,回肠最少,其体密度和数密度在第22周以前随胎龄增加而增大,之后则逐渐减小,在同一肠段肠腺内的CgA-IR细胞密度显著高于绒毛。各时期、各肠段CgA-IR细胞的平均截面面积和形状因子没有显著性不同。结论:人胚胎小肠CgA-IR 细胞自第7周出现后,其数量在肠段间、部位间有显著差异,其分布密度随胎龄而变化,以与其功能相适应。
Objective: To investigate the development of Chromogranin A-immunoreactive (CgA-IR) cells in human embryos. Methods: 40 cases of human embryonic small intestine CgA-IR cells from 6 to 38 weeks were subjected to stereological quantitative analysis by immunohistochemistry and stereological methods. Results: Human embryonic small intestine CgA-IR cells appeared in the seventh week of human embryo with the largest number of duodenum, followed by the jejunum with the least number of ileum. The body density and number density increased with the increase of gestational age before the 22nd week The density of CgA-IR cells in the intestine of the same intestine was significantly higher than that of the villi. In each period, the average cross-sectional area and shape factor of CgA-IR cells in each segment did not differ significantly. CONCLUSION: The number of CgA-IR cells in human embryonic small intestine is significantly different between intestine and intestine since the 7th week. The distribution density of CgA-IR varies with gestational age to adapt to its function.