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目的探讨纯钛钛片表面经不同方法处理对骨髓间充质干细胞矿化的影响。方法将直径为10mm,厚度为1mm,粗糙度为0.8μm纯钛钛片分三组进行表面处理:A组TiO2砂粒喷砂HCl/H2SO4混合液酸蚀组;B组Al2O3砂粒喷砂HCl/H2SO4混合液酸蚀组;C组未处理光滑组。通过SD大鼠骨髓间充质干细胞的体外培养与鉴定,将骨髓间充质干细胞培养于三组钛片表面并进行矿化诱导,采用扫描电镜观察三组纯钛片表面形貌及各组钛片表面矿化结节的形态,体视显微镜下计数矿化结节的数量。结果三组钛片表面矿化结节的数量之间均有统计学差异(P<0.05)。结论不同处理的钛表面对于骨髓间充质干细胞具有良好的相容性;TiO2砂粒喷砂酸蚀钛表面更有利于骨髓间充质干细胞的矿化。
Objective To investigate the effect of pure titanium on the mineralization of bone marrow mesenchymal stem cells by different methods. Methods Titanium titanium (10mm in diameter, 1mm in thickness and 0.8μm in roughness) was surface-treated in three groups: Group A sandblasting HCl / H2SO4 mixed acid etching group; Group B Al2O3 sandblasting HCl / H2SO4 Mixed solution etching group; C group untreated smooth group. Bone marrow-derived mesenchymal stem cells were cultured in vitro and induced by mineralization through the culture and identification of SD rat bone marrow-derived mesenchymal stem cells in vitro. Scanning electron microscopy was used to observe the surface morphology of the three groups of pure titanium and titanium Table mineralized nodules on the surface morphology, counting the number of mineralized nodules under the stereomicroscope. Results The numbers of mineralized nodules on the three groups of titanium surface were statistically different (P <0.05). CONCLUSION: The titanium surfaces treated by different treatments have good compatibility with bone marrow mesenchymal stem cells. The surface of titania sand-sprayed with TiO2 sand is more conducive to the mineralization of bone marrow mesenchymal stem cells.